| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236356 |
| Chromosome: | chromosome 1 |
| Location: | 6491745 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g046237 | GEX33 | (1 of 1) PTHR10799:SF777 - DNA-BINDING PROTEIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCCATGTATGATTTAGAAGCCGCTCAG |
| Internal bar code: | TTTTTCGAGTACGTCTCCTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 97 |
| LEAP-Seq percent confirming: | 53.8657 |
| LEAP-Seq n confirming: | 425 |
| LEAP-Seq n nonconfirming: | 364 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGACAAACTACACACCGA |
| Suggested primer 2: | GAGTGTGTGTGTGGTGGGAG |