| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236370 |
| Chromosome: | chromosome 11 |
| Location: | 2525954 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475950 | HLM16 | putative N-methyltransferase; (1 of 5) IPR001214//IPR003616 - SET domain // Post-SET domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGGGCATTTTGTGAAGGCTGCCTGGACA |
| Internal bar code: | CTATACGGGCTCATAGACGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 92.9231 |
| LEAP-Seq n confirming: | 302 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGCATGCCTCACATCTT |
| Suggested primer 2: | CATAATTGTCCCTCGACGCT |