| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236391 |
| Chromosome: | chromosome 12 |
| Location: | 2137080 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508850 | GST8 | (1 of 1) PTHR11260//PTHR11260:SF225 - GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING // SUBFAMILY NOT NAMED; Glutathione S-transferase, mitochondrial | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACTACAGCCTGCACGGGAGTTTTTCTT |
| Internal bar code: | GTTGGTTCTTCGGGTCACGCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 571 |
| LEAP-Seq percent confirming: | 98.6586 |
| LEAP-Seq n confirming: | 1471 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCGCGTGTGTGTTTACAT |
| Suggested primer 2: | ATAAGTGCCGGCTAGCTCAA |