| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.236434 |
| Chromosome: | chromosome 1 |
| Location: | 3623732 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023350 | THO2 | Oligoendopeptidase; (1 of 1) 3.4.24.16 - Neurolysin / Neurotensin endopeptidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGGCTTCTGCCACTGGCCCCAGGTGGCC |
| Internal bar code: | CTGCCGGGTGAAAGGCAGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 340 |
| LEAP-Seq percent confirming: | 99.5636 |
| LEAP-Seq n confirming: | 8213 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCTCAGGACCCGTACTT |
| Suggested primer 2: | GGACTGAGTCTCGGCATAGG |