| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.236444 |
| Chromosome: | chromosome 9 |
| Location: | 1785846 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396250 | VTE5 | Phosphatidate cytidylyltransferase; (1 of 1) K18678 - phytol kinase (VTE5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCTCCACAGTTCCATCCACACTACCCC |
| Internal bar code: | TTATAGTCTGGCAGGTTTACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 499 |
| LEAP-Seq percent confirming: | 95.5 |
| LEAP-Seq n confirming: | 191 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCCACTTCCTTAACCAAT |
| Suggested primer 2: | GCGACCTACCTCTCTGCAAC |