Insertion junction: LMJ.RY0402.236446_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.236446
Chromosome:chromosome 7
Location:2419012
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTGACGTCGCAACCCCGGCACCGGCGGC
Internal bar code:AAAGCGTCATCTCTCCCCCCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:346
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGCAAAGAAAGGTGGCTG
Suggested primer 2:CACAAGTATGCCCTGGGAGT