| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236446 |
| Chromosome: | chromosome 13 |
| Location: | 4565050 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603776 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGGTTAGTTAGTCCGGCGCGTGCGGCT |
| Internal bar code: | GCCGCGATCGCCTGCAGAGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 939 |
| LEAP-Seq percent confirming: | 99.0547 |
| LEAP-Seq n confirming: | 13517 |
| LEAP-Seq n nonconfirming: | 129 |
| LEAP-Seq n unique pos: | 192 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTCTACACAGTGCCCGTT |
| Suggested primer 2: | GCTCTGAACGGGTAGCAGTC |