Insertion junction: LMJ.RY0402.236460_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.236460
Chromosome:chromosome 14
Location:3848999
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g632767 (1 of 1) K01104 - protein-tyrosine phosphatase (E3.1.3.48) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAGGGTCAGCGTGCGCGCGTCTGATTGTA
Internal bar code:TGCCACTGGGTCATCACTACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:679
LEAP-Seq percent confirming:97.8261
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGTGGGGGTAGAAATGGT
Suggested primer 2:CTAGGTGCTAGGGAGGAGGC