Insertion junction: LMJ.RY0402.236488_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.236488
Chromosome:chromosome 9
Location:1411541
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAAAGTTTGTTCCTACCCCCCCCCTGTCG
Internal bar code:ATCGCTGCATGTCCGAGACTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1206
LEAP-Seq percent confirming:99.5947
LEAP-Seq n confirming:10811
LEAP-Seq n nonconfirming:44
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTACATGGGTGATCTGTG
Suggested primer 2:GGTTCGTGCCAAGTTTTGTT