| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.236497 |
| Chromosome: | chromosome 3 |
| Location: | 3445672 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g167250 | (1 of 1) PF05673 - Protein of unknown function (DUF815) (DUF815) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGCCGTGCCAACCCAGCCTCAAGTCCT |
| Internal bar code: | TCCAATTGATCGGGCGCGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 748 |
| LEAP-Seq percent confirming: | 99.4273 |
| LEAP-Seq n confirming: | 24307 |
| LEAP-Seq n nonconfirming: | 140 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTTTCGGGTGTGCTTAG |
| Suggested primer 2: | GACCGTCCCTTATAGCCACA |