| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236498 |
| Chromosome: | chromosome 10 |
| Location: | 4826637 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454025 | (1 of 1) PTHR23058//PTHR23058:SF0 - PEROXISOMAL MEMBRANE PROTEIN PEX14 // PEROXISOMAL MEMBRANE PROTEIN PEX14 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGGTAATGCATACATAGGAACAATGGA |
| Internal bar code: | GAGCAGTGAGAGGGAAATGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 99.2282 |
| LEAP-Seq n confirming: | 1800 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGCTGTAATACTCCCCGT |
| Suggested primer 2: | AGACATACGTCACCAATGCG |