| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236499 |
| Chromosome: | chromosome 2 |
| Location: | 4266096 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099000 | SYP71 | Qc-SNARE protein, SYP7-family; (1 of 2) K08506 - syntaxin of plants SYP7 (SYP7) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGGAAGAGTACCAGGAGGTGGAGGCAG |
| Internal bar code: | TCGTATCTAAACGCGAGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2968 |
| LEAP-Seq percent confirming: | 22.2222 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGAGTCGATCGGCACTA |
| Suggested primer 2: | ATCAGCTCCTTGATCTGCGT |