| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.236508 |
| Chromosome: | chromosome 3 |
| Location: | 5507213 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185800 | (1 of 14) PF00892 - EamA-like transporter family (EamA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTATCTTGCTTGCCTAGCGTTCATTCC |
| Internal bar code: | CCAAGAACTTTTTGTCAAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 10 |
| LEAP-Seq percent confirming: | 99.2366 |
| LEAP-Seq n confirming: | 130 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACTCATAAGTGGGACGCC |
| Suggested primer 2: | ACCTATCATGAGCACCGTCC |