| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236508 |
| Chromosome: | chromosome 16 |
| Location: | 498344 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692901 | (1 of 3) PTHR13748//PTHR13748:SF43 - COBW-RELATED // SUBFAMILY NOT NAMED; Putative metallochaperone | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTTTGACCTCTCATATCATTCCTCAGGA |
| Internal bar code: | GCAACGGTCCGACCGAGTGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 463 |
| LEAP-Seq percent confirming: | 97.7848 |
| LEAP-Seq n confirming: | 309 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTTCTTAGTCGTTCAGGC |
| Suggested primer 2: | CTTCGTGTACCAGGTGGGTT |