| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236509 |
| Chromosome: | chromosome 4 |
| Location: | 3646917 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228750 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTCGATGGTGGGATGGTGGGATAGTGG |
| Internal bar code: | TCGAGTCGAAGGCTGCGCGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 61.3811 |
| LEAP-Seq n confirming: | 240 |
| LEAP-Seq n nonconfirming: | 151 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCGTCCAATGGTGATGTA |
| Suggested primer 2: | ACTCAACTCCCTTTTCCGGT |