| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.236512 |
| Chromosome: | chromosome 10 |
| Location: | 4638523 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453050 | CYCM1 | (1 of 2) PTHR10026:SF51 - CYCLIN-K; Chlamydomonadale-specific cyclin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAACCTAAATGTGGAGACGCACGTCAGCC |
| Internal bar code: | GCATCTGTCACAGCATCGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 627 |
| LEAP-Seq percent confirming: | 99.4632 |
| LEAP-Seq n confirming: | 2038 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACTTCACCAGGGCACAGC |
| Suggested primer 2: | ATGTGCATGGACAATCATGG |