| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236514 |
| Chromosome: | chromosome 2 |
| Location: | 6169835 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113652 | (1 of 2) PTHR24320:SF1 - 3-KETODIHYDROSPHINGOSINE REDUCTASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAACACGTGCAATCAGTTCAGGGGCCCC |
| Internal bar code: | CGCGTACTGTCGGCACGTATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 99.7252 |
| LEAP-Seq n confirming: | 3266 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACACGGTAGACGCAATCC |
| Suggested primer 2: | GCAGGATAATGAAGGGCAAA |