Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.236518 |
Chromosome: | chromosome_13 |
Location: | 4607707 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g604050 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGGGTGCCGGCCAGGCGGGTGTGCGCATCA |
Internal bar code: | GGATGGGTTGGATGATCATTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 370 |
LEAP-Seq percent confirming: | 95.0254 |
LEAP-Seq n confirming: | 4680 |
LEAP-Seq n nonconfirming: | 245 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCATGTGGTGGTGGTGATG |
Suggested primer 2: | CATTTTCGTCTTTGGCGTTT |