Insertion junction: LMJ.RY0402.236518_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.236518
Chromosome:chromosome_13
Location:4607707
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g604050 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):AGGGTGCCGGCCAGGCGGGTGTGCGCATCA
Internal bar code:GGATGGGTTGGATGATCATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:370
LEAP-Seq percent confirming:95.0254
LEAP-Seq n confirming:4680
LEAP-Seq n nonconfirming:245
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ATCATGTGGTGGTGGTGATG
Suggested primer 2:CATTTTCGTCTTTGGCGTTT