Insertion junction: LMJ.RY0402.236532_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.236532
Chromosome:chromosome 2
Location:3311784
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095087 (1 of 2) 3.4.14.1 - Dipeptidyl-peptidase I / Dipeptidyl transferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCGTTTCCATTTCCGTTTCCGTTTCCGTT
Internal bar code:CTGGTAGGAACAAAGTCGCTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:619
LEAP-Seq percent confirming:98.5785
LEAP-Seq n confirming:3190
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCATCGTCAAGAACAGGA
Suggested primer 2:ACTACTATCGCCGGTCCCTT