| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.236536 |
| Chromosome: | chromosome 17 |
| Location: | 3077615 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g720900 | (1 of 100) IPR001810 - F-box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTTACCTGAGTGGCTGTGCCGTATGCT |
| Internal bar code: | CATGGCTCCAGTAAAGGCAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 461 |
| LEAP-Seq percent confirming: | 55.0 |
| LEAP-Seq n confirming: | 231 |
| LEAP-Seq n nonconfirming: | 189 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCACAGCTTTTCCTTGAT |
| Suggested primer 2: | CAACAGGAGAGCTGGCTACC |