Insertion junction: LMJ.RY0402.236540_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.236540
Chromosome:chromosome 13
Location:3734221
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACGGCGCGGAACCTGCGCGTGGGGAAGT
Internal bar code:GGTTTTTTCTGTCTGAGAGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1099
LEAP-Seq percent confirming:98.8696
LEAP-Seq n confirming:2274
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CAACAAGAACTACCGTCGCA
Suggested primer 2:GAGTGGCGCTACAGTGACAA