Insertion junction: LMJ.RY0402.236544_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.236544
Chromosome:chromosome 17
Location:3105261
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g721250 QilinDyf-3,FAP22,IFT38,Cluap1 (1 of 1) PF10234 - Clusterin-associated protein-1 (Cluap1); Intraflagellar Transport Protein 38 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCAATGTGCGCTGGGGTGAGCTGCAAGC
Internal bar code:TGCTTCCTTCTTCAGCTACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:666
LEAP-Seq percent confirming:99.4703
LEAP-Seq n confirming:939
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGGATGACGACGAGGATGAC
Suggested primer 2:ACAGTATCCAAGTCCGCTGC