| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236554 |
| Chromosome: | chromosome 16 |
| Location: | 414078 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTGGCGGCAGGCGCTTGCTGACGTGGA |
| Internal bar code: | TAATTGGCTGCCATGGTATTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 666 |
| LEAP-Seq percent confirming: | 99.479 |
| LEAP-Seq n confirming: | 8974 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGGGAAGCTCACTCTCT |
| Suggested primer 2: | ACCGACATGCAAACCTCTTC |