Insertion junction: LMJ.RY0402.236561_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.236561
Chromosome:chromosome 7
Location:1719358
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g325711 (1 of 2) K19363 - lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGACGGTACCGCATCCGTTCCCGCTTCTGC
Internal bar code:TAATCTGGATGCACCTCAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:325
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AACCTTTGGAATGAAACCCC
Suggested primer 2:CATGCTACAGTCTCCCAGCA