| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236561 |
| Chromosome: | chromosome 7 |
| Location: | 1719363 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325711 | (1 of 2) K19363 - lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTGCGCAGAGGGAGTCGTGTTTAAATT |
| Internal bar code: | GTGGGCCGCCGTAAAGACCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 879 |
| LEAP-Seq percent confirming: | 97.7972 |
| LEAP-Seq n confirming: | 2797 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 103 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTTTGGAATGAAACCCC |
| Suggested primer 2: | CATGCTACAGTCTCCCAGCA |