Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.236564 |
Chromosome: | chromosome_16 |
Location: | 6586259 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g672200 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCAGTGCGCTTTGAGGCGGTGGGCGTTGCC |
Internal bar code: | GCTACTAGGGCTCGCTCATGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 810 |
LEAP-Seq percent confirming: | 97.9275 |
LEAP-Seq n confirming: | 189 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCAACACGGTCTACGGAGG |
Suggested primer 2: | ACTCGTAGCTGACCTCGGAA |