| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236564 |
| Chromosome: | chromosome_16 |
| Location: | 6586266 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g672200 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGGCCGCGGCGCCAGCTGCCAAGGCACCC |
| Internal bar code: | CAGCAATGTCGTCGAGTTAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 98.8316 |
| LEAP-Seq n confirming: | 1438 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAACACGGTCTACGGAGG |
| Suggested primer 2: | CTTGGCTTTGGCGTCATAAT |