Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.236566 |
Chromosome: | chromosome_2 |
Location: | 4399333 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g099650 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGACGCTGCCGCGCACCCAGCAGCACCTTT |
Internal bar code: | GCGAGTGGACCCGGTTTGCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 755 |
LEAP-Seq percent confirming: | 99.5733 |
LEAP-Seq n confirming: | 4200 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCCACCTGACGAGAACTTG |
Suggested primer 2: | CATAACAGCGCAAACAGCAT |