| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.236575 |
| Chromosome: | chromosome 3 |
| Location: | 5229755 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182900 | (1 of 1) IPR008559 - Uncharacterised conserved protein UCP023322, transmembrane eukaryotic | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTGAGGCTGGAGCATGGCATTGACCGT |
| Internal bar code: | CTATGTTGCAGTCCGGCCCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 321 |
| LEAP-Seq percent confirming: | 99.7225 |
| LEAP-Seq n confirming: | 6108 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTCTCCAGCCACTGATAG |
| Suggested primer 2: | TGCAAGGTGTATGTGGGAAA |