| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.236578 |
| Chromosome: | chromosome_6 |
| Location: | 7002627 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g296700 | HYDG1,HYDG | Hydrogenase assembly factor/biotin synthase | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGGACTTGGACTCGGCGTTGACGGGCGCA |
| Internal bar code: | AGACCTTCCTAGCGGGACTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1211 |
| LEAP-Seq percent confirming: | 98.1333 |
| LEAP-Seq n confirming: | 1472 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCAACTCTTTCAGCCAGC |
| Suggested primer 2: | ACTAGCCAACCCCGAAGTTT |