| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.236585 |
| Chromosome: | chromosome 12 |
| Location: | 8603304 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547200 | PTK6 | Putative tyrosine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTATTTTAGCGTGCCAACCCATGCCGATG |
| Internal bar code: | TGGTGCACAAGCCCGCAAGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 99.3911 |
| LEAP-Seq n confirming: | 2122 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAAATGCCTAGAGCCTGG |
| Suggested primer 2: | GCTCACCTTGGCTGAGAAAC |