| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236588 |
| Chromosome: | chromosome 1 |
| Location: | 3612373 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023300 | (1 of 1) 1.1.1.370 - Scyllo-inositol 2-dehydrogenase (NAD(+)) | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGTGGGTGTGGGTGTTTGGGGGGCGGG |
| Internal bar code: | AAAACCTTCGGCAGGGTGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 98.4832 |
| LEAP-Seq n confirming: | 2727 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTCCATCCCTCCCTTCAC |
| Suggested primer 2: | GTCCTGACGGCTTACGTCTC |