| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.236590 |
| Chromosome: | chromosome 7 |
| Location: | 1389521 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322650 | CSA1 | Chlorophyceae-specific gene family A protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGGTTAATCCAGTGTGTGAGAGGCGGC |
| Internal bar code: | AATACGCCATGTGTGGCGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1358 |
| LEAP-Seq percent confirming: | 99.4033 |
| LEAP-Seq n confirming: | 18158 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGAGCATGCCTTCTCCTC |
| Suggested primer 2: | GCTTATCTAGCACGGCTTGG |