| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.236643 |
| Chromosome: | chromosome 12 |
| Location: | 7345309 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557252 | (1 of 1) K18985 - engulfment and cell motility protein 2 (ELMO2, CED12A) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGCGGTGTTTTGGCACCCCCTGCGTGTT |
| Internal bar code: | CGCGAGCTTACTGTTATGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 879 |
| LEAP-Seq percent confirming: | 99.5478 |
| LEAP-Seq n confirming: | 8145 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTAGGCGAGCCCTAGAAG |
| Suggested primer 2: | CACAATCATTGCCATTCAGG |