| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.236648 |
| Chromosome: | chromosome 4 |
| Location: | 3933907 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g231026 | SRP43,cpSRP43 | (1 of 1) K12271 - signal recognition particle 43 kDa protein (SRP43, CAO); 43 kDa subunit of chloroplast signal recognition particle | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCGCCCGCACCGCCGCCGCCTCATCCC |
| Internal bar code: | AAGCCAAGGTTGTCTGACAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 119 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGAAGGGGTCTTGGTGTA |
| Suggested primer 2: | CACCCCATTTCTTACACGCT |