| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.236685 |
| Chromosome: | chromosome 12 |
| Location: | 9691395 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556653 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGAGAAGACGGATTATTTGTGTGTGATT |
| Internal bar code: | ATCGGATGGGACGATGCGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 642 |
| LEAP-Seq percent confirming: | 99.5961 |
| LEAP-Seq n confirming: | 2466 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGACGACAAGGAGGAGGAG |
| Suggested primer 2: | TGTGTTACATTGCATGGGCT |