| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236711 |
| Chromosome: | chromosome 7 |
| Location: | 5947702 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354400 | CYP743B1,CYP21,CYP743B2,CYP743B3 | (1 of 3) 1.14.14.1//5.3.99.5 - Unspecific monooxygenase / Xenobiotic monooxygenase // Thromboxane-A synthase / Thromboxane synthetase; Cytochrome P450, CYP197 superfamily | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAAGACGGTGTTGCACAACCGTGTGCG |
| Internal bar code: | ATCACAGGACTCTGGACTTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 359 |
| LEAP-Seq percent confirming: | 99.5218 |
| LEAP-Seq n confirming: | 4787 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGATCTGGGTCTGCTACC |
| Suggested primer 2: | CTCAACCGGTAGGTTGCAGT |