| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236711 |
| Chromosome: | chromosome 8 |
| Location: | 4220769 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g380550 | SDR14 | (1 of 2) IPR002347//IPR013968 - Glucose/ribitol dehydrogenase // Polyketide synthase, ketoreductase domain; Short-chain dehydrogenase/reductase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTGGTGCTGCTGTGTTCGATGTATGGG |
| Internal bar code: | ACCCCAACTATTGCGCGATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 605 |
| LEAP-Seq percent confirming: | 98.8479 |
| LEAP-Seq n confirming: | 3346 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGGCTTATAACTGCTGC |
| Suggested primer 2: | CTCGCACTTCAATCCTAGCC |