| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.236718 |
| Chromosome: | chromosome 7 |
| Location: | 3700407 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337582 | (1 of 1) PF03448 - MgtE intracellular N domain (MgtE_N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTCTCCACCAGCCCCATCAGCCAGCCC |
| Internal bar code: | CGTGTTCCGGGCTCACCGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 116 |
| LEAP-Seq percent confirming: | 98.9796 |
| LEAP-Seq n confirming: | 97 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCCTCTGTGTGTTTGGG |
| Suggested primer 2: | AGCAGCAGGAGCTGAGTGAC |