| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236738 |
| Chromosome: | chromosome 11 |
| Location: | 3024316 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGAAGTGGCAGCAGCAGGAGGGCAGCA |
| Internal bar code: | GCGTTTCGATCGATGAAACAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 623 |
| LEAP-Seq percent confirming: | 98.8515 |
| LEAP-Seq n confirming: | 1205 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTAGGAAGGCTGGGGAAAT |
| Suggested primer 2: | CGCTTTCTGCCTATCTCACC |