| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236747 |
| Chromosome: | chromosome 4 |
| Location: | 2385536 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221050 | (1 of 3) PTHR12136//PTHR12136:SF47 - STEROIDOGENIC ACUTE REGULATORY PROTEIN STAR // STEROIDOGENIC ACUTE REGULATORY-LIKE PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTACGCATGCATCCAAGCGATAGTGCGA |
| Internal bar code: | GAACGCCCAAACGTTCCCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 99.552 |
| LEAP-Seq n confirming: | 3333 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGTGTGCCACCTAGTTCC |
| Suggested primer 2: | TACAAGCGCAATCGTGAAAG |