| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.236752 |
| Chromosome: | chromosome_10 |
| Location: | 2938953 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g440350 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCATGAACCGCGTGTAGCTTCATCAAAAT |
| Internal bar code: | CTTCTATTGTACCGTCATCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 612 |
| LEAP-Seq percent confirming: | 99.0146 |
| LEAP-Seq n confirming: | 3115 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACCCACCGACAGTTCTTA |
| Suggested primer 2: | CTTGCCTCTGGGACTCACTC |