Insertion junction: LMJ.RY0402.236753_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.236753
Chromosome:chromosome 16
Location:2058184
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTTCGCACATGCAAGGCGCGCGCCGCGC
Internal bar code:CGCGGAGCTGGAGCTACGGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:557
LEAP-Seq percent confirming:99.2011
LEAP-Seq n confirming:1490
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTTGCTCTCCTCCAGTCC
Suggested primer 2:GAATTCGGTCTGCAGGAGAG