| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.236772 |
| Chromosome: | chromosome 13 |
| Location: | 4500966 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602400 | KIN8A,KIN8-1 | Kinesin motor protein; (1 of 1) K10401 - kinesin family member 18/19 (KIF18_19) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGACACGATGCACCGGGATGAGGAGTTCT |
| Internal bar code: | GTGGGGGTGGAGAACTAGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 430 |
| LEAP-Seq percent confirming: | 98.6708 |
| LEAP-Seq n confirming: | 965 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCAGCAAGCAGAATACA |
| Suggested primer 2: | CGTGATCTCAAGCACCTGAA |