Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.236778 |
Chromosome: | chromosome_3 |
Location: | 2326551 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g158400 | FAP218 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACGTTGCTCCTCAAGCTGCGGCTCTCGTCC |
Internal bar code: | TTACCGATGGTTCAGCTCCGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 204 |
LEAP-Seq percent confirming: | 95.8549 |
LEAP-Seq n confirming: | 185 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTGGCACTGAATGACGTTG |
Suggested primer 2: | TCGTCATGTGTGTCATGTGG |