| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236784 |
| Chromosome: | chromosome 4 |
| Location: | 3463075 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g227750 | (1 of 1) PF08573 - DNA repair protein endonuclease SAE2/CtIP C-terminus (SAE2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTGCGTGGAGACACCAAGGGTGGTGCG |
| Internal bar code: | GTTACGTGCGCCGCCGGTAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 467 |
| LEAP-Seq percent confirming: | 98.928 |
| LEAP-Seq n confirming: | 646 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTCATGACCACAACCAC |
| Suggested primer 2: | CTGATGACACCACGTCTGCT |