| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236793 |
| Chromosome: | chromosome 3 |
| Location: | 4767386 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178200 | CCT10 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGCGCTCGCCCTCCTGGCTGGGTGCGC |
| Internal bar code: | ATAGATAGTGTGGACATTGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 91.6279 |
| LEAP-Seq n confirming: | 197 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAACTCCAGCCTTCAAGC |
| Suggested primer 2: | GATTGCTGCTCTCAACTCCC |