| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236807 |
| Chromosome: | chromosome 8 |
| Location: | 358547 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358571 | (1 of 5) IPR001054//IPR006189//IPR029787 - Adenylyl cyclase class-3/4/guanylyl cyclase // CHASE domain // Nucleotide cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCACCTGGACACCCCACTTGCCACGGC |
| Internal bar code: | ATTCACACTTTAGATTTGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 468 |
| LEAP-Seq percent confirming: | 99.3471 |
| LEAP-Seq n confirming: | 3500 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGAGAGTGTGAGCACTGA |
| Suggested primer 2: | CCCACTGTACCGACACACAC |