| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.236824 |
| Chromosome: | chromosome 11 |
| Location: | 793744 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467639 | (1 of 1) PF04910 - Transcriptional repressor TCF25 (Tcf25) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATGCCGCTCGGAAGCCCTCACCACAAA |
| Internal bar code: | TTGTGGTGTTCCCTAGTCGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 368 |
| LEAP-Seq percent confirming: | 99.0846 |
| LEAP-Seq n confirming: | 3572 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTACCGGTTATGAGGGG |
| Suggested primer 2: | ATGTGTGTGATGTGCGTGTG |