| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236833 |
| Chromosome: | chromosome 12 |
| Location: | 7435036 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCTTCCACCAGACCTACTTCAACCAGAC |
| Internal bar code: | GTACGTGCGTGGCCTAGGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 97.8333 |
| LEAP-Seq n confirming: | 587 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGTGCTGTTCGGGTAAT |
| Suggested primer 2: | GTGGCTGCTTTCCTGATCTC |